Composite
Part:BBa_K1132022:Experience
Designed by: iGEM Toulouse Group: iGEM13_INSA_Toulouse (2013-09-20)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Results of BBa_K1132022 characterization
After 18 hours, clones containing the plasmid (BBa_J23116-TetR-pTet-RFP) show a leaky basal expression of mRFP. We suppose that the promoter was too weak to express TetR in large quantity. Assembly of a stronger promoter could improve the system and lock the response to an ON/OFF response.
Besides, an experience was done to analyze the effect of the aTc inducer. Result show a visible induction of the red fluorescent protein expression by addition of aTC (60 ng/mL).
User Reviews
UNIQ1ffdb22ba5e861e2-partinfo-00000000-QINU UNIQ1ffdb22ba5e861e2-partinfo-00000001-QINU